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Fig. 1. Cell cycle arrest at the G1-S phase boundary caused by iron chelators. (A) Flow cytometry analysis of propidium iodide stained nuclei, isolated from HeLa cells that were treated for 24 hours with the iron chelators mimosine, ciclopirox olamine or 2,2'-bipyridyl, and, as control, with the replication-elongation inhibitor aphidicolin, at the indicated concentrations. The positions of unreplicated (2n) and fully replicated DNA content (4n) are indicated. (B) In vitro replication reactions of nuclei, isolated from cells treated with the iron chelators and aphidicolin as in A. Nuclei were incubated in an elongation buffer (`buffer', light grey bars) or in the same buffer supplemented with 100 µg HeLa cell cytosolic extract (cytosol, dark grey bars). Percentages of nuclei that incorporated labelled nucleotides were determined. The mean of 3-4 experiments and the standard deviations are shown.
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