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Fig. 7. Nuclear lamina organization in klp61F mutant spermatocytes. Testes from wild type (A-C) and klp61F3 mutants (D-F) stained with antibodies against {gamma}-tubulin (red), lamin (green) and a fluorescent chromatin dye (blue). Images are single projections of a stack of optical sections. (A) Antibodies against {gamma}-tubulin crossreact with a mitochondrial antigen(s) in testes, providing a useful marker for mitochondrial derivatives and the progression of spermatocyte development. (E,F) Arrows indicate acentrosomal spermatocytes. (E) To show the apposition of centrosomes and chromatin relative to invaginations of nuclear lamina, the lasso tool of Photoshop was used to replace the projected stack of lamin staining with lamin staining in a single optical section in the spermatocyte outlined in dots. Bar, 5 µm.





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