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Figure 8


Fig. 8. Micronuclei in klp61F ncd spermatocytes at telophase. Testes in klp61F ncd mutants (A-F), wild-type animals (G) and ncd1 mutants (H,I) were stained with antibodies against {gamma}-tubulin (green) and a fluorescent chromatin dye (blue) and {alpha}-tubulin (green, A-C) or lamins (green, D-I). (A,D) Multiple nuclei were not detected in metaphase of meiosis, but were detected at the poles of telophase spindles (B, arrows) and in spermatids (C,F arrows). Multiple nuclei were not detected in wild-type (G) or in ncd1 (H) spermatocytes in telophase of meiosis or in post-meiotic spermatocytes (I). Bar, 2 µm.





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