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Figure 9


Fig. 9. Ncd localization in klp61F and ncd mutant spermatocytes. Spermatocytes in wild type (A-D), ncd1 (E) and klp61F3/klp61F1 (F) mutants stained with a blue fluorescent chromatin dye and antibodies against Ncd (red), {gamma}-tubulin (green, A,C,E), {alpha}-tubulin (green, B,D) and lamin (green, F). Arrows in (A) indicate enriched Ncd near heterochromatin in primary spermatocyte. (C) Arrow indicates overexposure of nuclear staining of Ncd in primary spermatocyte for comparison with spindle staining marked by asterisks. (F) Arrow indicates lamina around bivalent in spermatocyte near prometaphase. Bar, 5 µm.





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