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Fig. 3. Loss of WAL1 leads to increased isotropic growth and defects in septation. (A) Wild-type hyphae showed accumulation of chitin at septal sites (arrowheads) and more intensely stained hyphal apices (brackets). (B,C) The wal1 hyphae were stained uniformly and rarely displayed chitin-rich septa. Notice the swellings of wal1 hyphae and the short distance between consecutive dichotomous tip branches. Staining was done using calcofluor. Scale bar, 20 µm.





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