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Fig. 5. PMN-mediated degradation of Nvj1p is unperturbed in osh1 ${Delta}$ cells. (A,B) Starvation-induced degradation of Nvj1p-Myc is dependent on VAC8 and PEP4 but not ATG7 or OSH1. The turnover of a limited pool of Nvj1p-Myc during nitrogen starvation was analysed by immunoblot in isogenic wild-type, atg7 ${Delta}$ and vac8 ${Delta}$ cells (A) or osh1 ${Delta}$ and pep4 ${Delta}$ cells (B). The amount of Nvj1p-Myc remaining after 10 hours in SD-N was digitally quantified and compared with the level before starvation. Bar graphs represent the average percentage loss of Nvj1p-Myc from several independent experiments.

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