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Fig. 1. Intracellular redistribution of the various cPKCs isoforms in mouse oocytes during the period preceding germinal vesicle breakdown (GVBD). (A) The photographs show the three different types of immunolabeling observed with the four antibodies, against PKC-, PKC-ßI, PKC-ßII and PKC-, by confocal microscopy of whole mouse oocytes during spontaneous resumption of meiosis. These observations were recorded for a single optical section through the GV. Type I: spots of dense staining in the cytoplasm, intense labeling around the nuclear envelope, no staining in the nucleoplasm; type II: increased labeling in the nucleoplasm; type III: considerable immunoreactivity almost exclusively within the nucleus and around the cortex; isolated spots of fluorescence scattered throughout the cytoplasm. Scale bar, 40 µm. (B) The histograms indicate the distribution of the three different types of immunostaining with respect to time after meiosis reinitiation for each cPKC isoform. White columns: type I; gray columns: type II; black columns: type III. (C) The photographs show the immunolabeling of whole mouse oocytes, observed with the antibodies against PKC-, PKC-ßI and PKC-ßII when the meiotic arrest was maintained by hypoxanthine during 1 to 3 hours.

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