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Fig. 2. Temporal patterns of staining with the various anti-cPKC antibodies in isolated nuclei. For each cPKC studied, the selected photographs are representative of the redistribution of immunostaining observed by confocal microscopy in isolated nuclei. The nuclei were isolated from oocytes cultured for 30 or 90 minutes and then immediately immunostained with an antibody against one of the cPKCs studied. The bottom panel shows control nuclei immunostained with an antibody against lamin A/C. (Left) The nuclear envelope is retained during the nuclear isolation procedure. (Right) Corresponding transmission image of the nucleus. Bar scale, 10 µm.





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