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Fig. 2. Subcellular localisation of Cdc25B. (A) Representative images of Cdc25B-staining using the B1:5 antibody. Cells were co-stained with cyclin B1 antibodies and Hoechst 33342 was used to stain nuclei. (B) Nuclear and cytoplasmic Cdc25B levels increase as cyclin B1 accumulates. The fluorescence signal from a deconvoluted image was quantified, the average background from cells not expressing Cdc25B was subtracted and the quantified B1:5 signal (y-axis) was plotted against the quantified cyclin B1 signal (x-axis). Each point in the graph corresponds to one cell. (C) Cdc25B immunofluorescence of cells at different stages of mitosis.





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