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Fig. 3. Cdc25B nuclear export is mediated by a region between amino acids 54 and 67. (A) Schematic presentation of the N-terminus of Cdc25B. (B) Localisation patterns of EYFP fusion proteins containing parts of the Cdc25B N-terminus as indicated, in the presence or absence of leptomycin B (LMB). hTERT immortalized human fibroblasts were microinjected with constructs expressing parts of the Cdc25B N-terminus fused to YFP. During the last 4 hours before fixation half of the cells were incubated with leptomycin B. (C) Nuclear export of GST Cdc25B 1-67. Purified GST-Cdc25B was microinjected together with Texas Red-conjugated dextran into the nucleus of hTERT immortalized human fibroblasts. One hour later, cells were fixed and stained with anti-GST antibodies (green).
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