spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Induction of premature mitosis by overexpressing Cdc25B mutants. Cells were synchronised by a double thymidine-block, transfected with YFP-Cdc25B3, YFP-Cdc25B3(L62A), YFP-Cdc25B3(S323G), YFP-Cdc25B3(NLS-) or YFP alone immidiately after release and fixed after 8 hours. The different constructs were expressed at similar levels as determined by the intensity of YFP fluorescence. Cells expressing the constructs were counted and scored for premature chromatin condensation (% PCC) as judged by Hoechst 33342 staining. Data show the mean of three independent experiments. Images on the right show examples of a typical cell displaying prematurely condensed chromatin (a) and an interphase cell (b).





Right arrow Return to article