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Fig. 7. UIM-WT, but not UIM-mut, inhibits EGF-induced re-distribution of endogenous Eps15. CHO cells expressing EGFR-WT together with an empty vector (A-D), FLAG-UIM WT (E-H) or FLAG-UIM-mut (I-L) were incubated with EGF-TxR (red) on ice for 1 hour. After stimulation at 37°C for 1 minute (A-C, E-G, I-K) or 5 minutes (D,H,L), cells were stained with anti-Eps15 pAb, followed by FITC-conjugated secondary antibodies (green). Cells were analyzed by confocal microscopy. The displayed confocal planes are from the basal half of the cells, except for panel (L), which is from the upper part of the cell. XZ sections are shown below each panel. (M) For each transfectant used in the confocal experiment described above, 3x50 cells were analyzed at t=1 minute and scored for plasma membrane recruitment of Eps15. Black bars, control cells; grey bars, UIM-WT-expressing cells; white bars, UIM-mut-expressing cells. Error bars represent standard deviations between the three independent analyses.

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