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Fig. 2. The presence of ddFIP mRNA and protein during development. Dictyostelium amoebae of strain AX2 were starved on phosphate agar. At the indicated time points, samples were taken for RNA extraction or preparation of total cell homogenates. (A) RNA blots containing 30 µg of total RNA per lane were probed with a 2.7 kb fragment encompassing the 5'-end of the FIP DNA. Transcript levels are rising during the early developmental stages and are maintained throughout culmination. During maturation of the fruiting body, the transcript level decreases. (B) Total cell homogenates of 1x106 cells per lane were resolved in 8% polyacrylamide gels and blotted onto nitrocellulose membranes. Blots were incubated with mAb K12-454-2. The antibody recognizes a 230 kDa band throughout all stages of development.





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