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Fig. 6. Localization of the GFP-cFIP fusion protein during phagocytosis and exocytosis. Single confocal sections through living AX2 cells expressing the GFP-cFIP fusion protein. (A) Uptake of TRITC-labeled yeast cells (indicated by a star). GFP-cFIP is located at the plasma membrane during the formation (upper panel) and the engulfment of the phagosome (lower panel). GFP-cFIP is released from the phagosomal membrane after complete engulfment of the yeast particle. (B) GFP-cFIP reassembles at the membrane during exocytosis of TRITC-labeled yeast cells. Bar, 5 µm.





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