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Fig. 2. Relative Rad18Sc protein level in mouse testis at different postnatal developmental time points and in isolated germinal cells. (A) Total testis proteins from mice at different postnatal ages (7-36 days) were analyzed on an immunoblot (WB) using anti-Rad18Sc. The relative Rad18Sc protein level increases after birth to a maximum at 21 days. (B) The Rad18Sc protein level in isolated spermatocytes (scy) is slightly higher than in isolated spermatids (rst). Ponceau S staining of total protein (TP) is shown as a loading control.