QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 9. RNA polymerase II is largely excluded from nuclear regions containing Rad18^Sc associated with unsynapsed chromosomes. (A) Left: Immunostaining of the SC using anti-Sycp3 (red) on a wild-type spermatocyte. The arrow points to the XY body. Right: Coimmunostaining of Sycp3 and RNA polymerase II (RNA pol II, green) showing that the RNA polymerase II staining signal is very low in the XY body. (B) Left: Immunostaining of Sycp3 (red) on a T(1;13)70H/T(1;13)Wa double-heterozygous spermatocyte. The arrow points to the XY body and the arrowhead to the 1¹³ translocation bivalent. Right: Co-immunostaining of Sycp3 and RNA polymerase II (RNA pol II, green) shows that the RNA polymerase II staining signal is also very low in the subnuclear region of the 1¹³ translocation bivalent. (C) Left: Immunostaining on a T(1;13)70H/T(1;13)Wa double-heterozygous spermatocyte with anti-RNA polymerase II (RNA pol II, green). The subnuclear regions containing the XY body (arrow) and the 1¹³ translocation bivalent (arrowhead) have a very low level of RNA polymerase II protein. Right: Co-immunostaining of RNA polymerase II and Rad18^Sc shows that the regions deficient in RNA polymerase II contain a large amount of Rad18^Sc (red). Bar, 20 µm.

Return to article