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Fig. 2. Full-length Dronc is cleaved by active Dronc at E352 and by active Drice at D135. (A) Full-length in-vitro-translated wild-type (WT) or E352A Dronc was incubated with active recombinant Dronc, Drice or both at 30°C for 1 hour and analysed by SDS-PAGE and autoradiography. (B) Full-length in-vitro-translated D113A, D135A, D113A/D135A or D133A/D135A/E352A Dronc was incubated with active recombinant Dronc, Drice or both at 30°C for 1 hour. Reactions were then separated by SDS-PAGE and examined by autoradiography. (C) The three processed forms of Dronc: FL, full-length Dronc; L, Dronc large subunit; P, Dronc prodomain. The asterisk indicates a Dronc cleavage product resulting from cleavage of full-length Dronc by Drice at D113.





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