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Fig. 3. Processing at E352 is required for Dronc activity in vitro. Recombinant wild-type or E352A Dronc was incubated in caspase buffer A alone (filled bars) or in caspase buffer A plus S2 cell lysate (empty bars) at 30°C for 30 minutes. IETD-afc was then added to both reactions, which were then incubated for another 30 minutes, and activity was determined by comparing fluorescence units. The results shown are representative of three independent experiments.





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