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Fig. 1. Identification of SmAV as a CaP interacting protein. (A) Interactions between SmAV and CaP in reciprocal yeast two-hybrid assays. The growth of yeast host (YRG2) with plasmids or constructs in Trp-, Leu- and His-depleted selective medium. (1) Yeast Host-YRG2. (2) pBD-GAL4 Cam. (3) pBD-GAL4 Cam-CaP. (4) pBD-GAL4 Cam-CaP and pAD-GAL4-2.1-c-SmAV. (5) pBD-GAL4 Cam-c-SmAV. (6) pBD-GAL4 Cam-c-SmAV and pAD-GAL4-2.1-CaP. (7) pBD-GAL4 Cam-c-SmAV and pAD-GAL4-2.1-Cap136. (8) pBDGAL4 Cam-c-SmAV and pAD-GAL4-2.1-CaP163. (B) CaP domains indicating the amino acid residue numbers (ferret sequence) and the domains (Morgan and Gangopadhyay, 2001). Double-headed arrows indicate the deletion constructs used. (C) Expression of LacZ as detected by blue color development with X-gal. Colonies of yeast cells (either host only or transfected with plasmid/constructs) are numbered as in (A).





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