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Fig. 5. Co-transfection with CaP prevents extraction of EGFP-c-SV by Triton X-100 from COS-7 cells. (A, left) Unextracted COS-7 cell singly transfected with EGFP-c-SV. (A, right) COS-7 cell singly transfected with EGFP-c-SV and extracted with 0.5% Triton X-100 before fixation. (B, left) Unextracted COS-7 cell singly transfected with CaP. (B, right) COS-7 cell singly transfected with CaP and extracted with 0.5% Triton X-100 before fixation. (C, left) Unextracted COS-7 cell co-transfected with EGFP-c-SV (top) and CaP (middle). (C, right) COS-7 cell co-transfected with EGFP-c-SV (top) and CaP (middle) and extracted with 0.5% Triton X-100 before fixation. Merged images shown at the bottom, with regions of overlap in yellow. Scale bar, 10 µm.
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