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Fig. 7. CaP and SmAV colocalize in an agonist-dependent manner in differentiated aortic smooth-muscle cells. (A) Western blot of aorta tissue homogenate probed with anti-H340 antibody. Molecular weight standards are indicated on the left. (B) Confocal images of a freshly isolated ferret aorta cell fixed under control conditions and labeled for endogenous levels of CaP (left, green) and SmAV (middle, red). Merged images are shown on the right. (C) Confocal images of a freshly isolated ferret aorta cell fixed in the presence of 10-5 M phenylephrine for 10 minutes and labeled for CaP and SmAV. Merged images are on the right, with signal overlap in yellow. Scale bar, 10 µm.





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