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Fig. 8. Quantification of subcellular distribution of CaP and SmAV in differentiated smooth-muscle cells. Fluorescence intensities were quantified from confocal micrographs. In all cases, between 14 and 53 cells were analysed. (Inset) Method used to produce line scans for quantification of ratios. (A) Comparison of the average (with standard error) of surface:core ratios for the distribution of SmAV in the absence and presence of phenylephrine (PE). (B) Comparison of the average surface:core ratios for the distribution of CaP in the absence and presence of PE. (C) Average ratios (+ s.e.m.) of SmAV surface-membrane to cytosol fluorescence intensities in single smooth-muscle cells that were unstimulated or stimulated with PE for 2 minutes, 4 minutes or 10 minutes. ****P<0.0001 compared with unstimulated values.





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