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Fig. 1. ST6GalNAc I transfectants express cell surface STn epitopes. (A) Cell surface glycosylation was analyzed by flow cytometry using the anti-STn mAbs TKH2 and B72.3 as well as the PNA and JAC lectins that recognize preferentially the O-glycan core 1. Control clones B10 and F4 correspond to parental and mock-transfected cells, respectively. Clones C2 and G9 are ST6GalNAc I transfectants. The log of fluorescence intensities in arbitrary units is plotted against cell number. Fluorescence intensities from negative controls performed in the presence of either an irrelevant antibody or FITC-labeled streptavidin alone are shown superimposed in dotted lines. (B) Sialyltransferase activities from total cell extracts were assayed using fetuin as acceptor and CMP-[14C]NeuAc as sugar donor as described in Materials and Methods. Specific activities are given as pmol/h/mg total proteins of [14C]NeuAc transferred. (C) Expression of STn epitopes was observed on fixed and permeabilized cells by confocal microscopy using the TKH2 antibody on the C2 clone.





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