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Fig. 3. The ability of ST6GalNAc I transfectants to repair a scratch wound and to proliferate is impaired. (A) Photomicrographs were taken 24 hours after performing scratch wounds on confluent cell monolayers of a STn- and a STn+ clone. (B) Wound repair was quantified by image analysis on the STn-control clones B10 and F4 (blue symbols) and the STn+ clones C2 and G9 (red symbols). The wound breadth was measured at different time points after wounding, and results represent the mean of three independent experiments. Mean speed of wound repair, 13.3±1.1 and 6.28±1.2 µm/hour for STn- and STn+ cells, respectively (P<0.001). (C) Cell proliferation was measured by a neutral red uptake assay as described in Materials and Methods. Error bars represent the standard error of triplicates within one out of two experiments.
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