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Fig. 6. Treatment with anti-STn and anti-integrin restores the motility of ST6GalNAc I transfectants on fibronectin. Motility was assessed by a phagokinetic track assay on immobilized fibronectin. Before seeding, cells were incubated with antibodies for 30 minutes at 37°C. (A) Cells were treated with the anti-STn mAb TKH2 and the irrelevant control IgG1 at the optimal concentration of 1 µg/ml. The values correspond to the mean ± standard deviation of five independent experiments. (B) Cells were pretreated with the anti-{alpha}5 and ß1 integrin subunits 5H10-27 and KMI6, respectively, at the concentrations that gave maximal effect (25 µg/ml and 5 µg/ml, respectively). Values represent the mean ± standard deviation of three independent experiments.





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