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Fig. 1. Schematic of the membrane dipeptidase (MDP) constructs used. Wild-type porcine MDP (wtMDP) contains an N-terminal 16 amino acid signal sequence (diagonally hatched box), two N-linked glycosylation sites (N41 and N263, lollipops) and a C-terminal GPI anchor addition signal (chequered box). The GPI anchor is attached to S368. In MDP{Delta}GPI and MDP{Delta}GPI{Delta}gly, A369 was mutated to a stop codon, resulting in secreted proteins. In MDP{Delta}gly and MDP{Delta}GPI{Delta}gly, N41 and N263 were mutated to Gln to prevent N-glycosylation.





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