spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Expression and enzymatic activity of the MDP constructs in MDCK cells. Confluent monolayers of MDCK cells stably expressing wtMDP (wt), MDP{Delta}GPI ({Delta}GPI), MDP{Delta}gly ({Delta}gly), MDP{Delta}GPI{Delta}gly ({Delta}GPI{Delta}gly) or empty vector (pCI) were incubated in OptiMEM for 7 hours. The conditioned medium was harvested and membranes prepared from the cells as described in Materials and Methods. (A) Membranes (10 µg protein per lane) and medium (40 µg protein per lane) were subjected to western blot analysis with an antibody against porcine MDP. Results are representative of three independent experiments. (B) MDP activity was quantified using the selective substrate Gly-D-Phe with the product D-Phe being separated and quantified by reverse phase HPLC (Hooper et al., 1987). The data are the mean±s.e.m. of three determinations.





Right arrow Return to article