spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Glycosylation and glycosylphosphatidylinositol (GPI) anchorage of the MDP constructs. (A) Membranes (10 µg of protein) from MDCK cells stably expressing either wtMDP (wt) or MDP{Delta}gly ({Delta}gly) or medium (40 µg of protein) from cells expressing MDP{Delta}GPI ({Delta}GPI) or MDP{Delta}GPI{Delta}gly ({Delta}GPI{Delta}gly) were incubated in the absence or presence of peptide N-glycosidase F (PNGase F) for 16 hours. The samples were then subjected to western blot analysis with anti-MDP antibody. The results are representative of three independent experiments. (B-E) Cells transfected with either wtMDP (B,D) or MDP{Delta}gly (C,E) were incubated in the absence (B,C) or presence (D,E) of 150 mU/ml B. thuringiensis PI-PLC, then fixed in paraformaldehyde and subjected to immunocytochemistry using the polyclonal anti-MDP antibody. The images are representative of three independent experiments. Bar, 20 µm.





Right arrow Return to article