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Fig. 6. Rickettsia conorii infection of Vero cells induces the tyrosine phosphorylation of pp125FAK. (A) Pre-incubation of Vero cells with the indicated inhibitors of bacterial invasion, blocks the induced tyrosine phosphorylation of at least one host protein (p125/130) after 5 minutes of bacterial infection (arrow). (B) Immunoprecipitation of pp125FAK and immunoblot analysis with anti-phosphotyrosine (pTyr) demonstrates an induced phosphorylation of FAK during the infection process, suggesting that this protein may play a key role in the entry process.





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