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Fig. 2. Expression of myr-HA-ß₂AR-C in Xenopus oocytes and in COS-7 cells. COS-7 cells mock transfected (-) or transfected with myr-HA-ß₂AR-C (+) were lysed and analyzed by immunoblotting with antibodies against HA (left panel). Frog oocytes were injected with water (-) or mRNA for myr-HA-ß₂AR-C (+). Oocytes injected with myr-HA-ß₂AR-C mRNA were incubated in OR2 (-) or with progesterone for the indicated periods. Oocytes were lysed and analyzed by immunoblotting with antibodies against HA (right panel). Oocytes treated overnight (o/n) with progesterone (Pg) exhibited 100% germinal vesicle breakdown (GVBD). The arrow indicates the position of myr-HA-ß₂AR-C and the asterisk indicates a nonspecific protein, that apparently exists in both frog oocytes and in COS-7 cells.

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