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Fig. 3. Progesterone-induced dephosphorylation of myr-HA-ß₂AR-C. (A-J) Xenopus oocytes injected with the indicated mRNAs were incubated in OR2 for 24 hours. Groups of at least 20 oocytes were incubated in OR2 or OR2 containing 1 µM progesterone (Pg). Group D received 50 µM forskolin (Fsk) 20 minutes prior to the addition of progesterone. One hour later, oocytes were lysed and analyzed by 2D gel electrophoresis followed by HA immunoblotting. Shown are representative examples of three to ten independent experiments. The long dashed line separates two first dimension mini-tube gels. Shown in the PKA^-&GRK^- panel (on the right side of panel H) is one example of an extra SDS sample directly loaded on the second-dimension gel. OH^- and H⁺ denote the anion and cation ends, respectively, of the tube gels. Circles represent positions of missing spots (1 or 2). Arrow, myr-HA-ß₂AR-C; asterisk, nonspecific protein.

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