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Fig. 4. Protein kinase A phosphorylated myr-HA-ß2AR-C in Xenopus oocytes. (A-D) Oocytes injected with mRNA for myr-HA-ß2AR-C were incubated in OR2 for 24 hours. One group received a further injection of 10 nl PKAc per oocyte. One hour after the PKAc injection, oocytes were incubated in OR2 or OR2 containing H89 (100 µM) or progesterone (Pg), as indicated. One hour later, 20 oocytes were retrieved from each group for lysis and 2D electrophoresis and immunoblotting with HA antibodies. Circle in (D) represents the position of missing spot 1. Arrow, myr-HA-ß2AR-C; asterisk, nonspecific protein. (E) The remaining oocytes in each group were scored for GVBD at the indicated time following the addition of H89 or progesterone. Only the H89 data are shown here (representative of three independent experiments).





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