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Fig. 5. A time course of myr-HA-ß2AR-C dephosphorylation caused by progesterone. (A-D) Xenopus oocytes injected with mRNA for myr-HA-ß2AR-C were incubated for 24 hours. Twenty oocytes were set aside as control (OR2) and the rest were incubated with progesterone (Pg). At each of the indicated times, 20 oocytes were removed and all were then subjected to 2D electrophoresis and HA immunoblotting. (D) Multiple phosphorylated forms of myr-HA-ß2AR-C were seen in oocytes that had undergone GVBD (represented by X). (E) Progesterone-treated oocytes were scored for GVBD. The same lysates were also analyzed for histone H1 kinase (MPF) activities (H1) and for MAP kinase phosphorylation (p-MAPK). Representative examples from three independent experiments are shown.





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