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Fig. 7. YP170 and yolk receptor RME-2 accumulation in Caenorhabditis elegans cpl-1(ok360) mutant embryos. (A-F) Immunofluorescence with antibody to YP170 (red) and RME-2 (green) in wild-type (A-C) and cpl-1 mutant (D-F) embryos. Localisation of RME-2 to the periphery of enlarged yolk vesicles is seen in cpl-1 mutant embryos (F), but not in wild-type embryos (C). (B,C) The embryo on the right shows anti-RME-2 localisation in a one-cell stage wild-type embryo, prior to RME-2 degradation. (G,H) IEM localisation of RME-2 antibody to small vesicles in wild-type embryo (G) and to electron-light regions at the periphery of enlarged yolk vesicles in cpl-1(ok360) embryo (H). Bar, 500 nm.





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