|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. MECs secrete immature and mature forms of CD; BFA does not completely abolish this secretion. (A) Immunofluorescence localisation of CD and TGN38 (as a marker of Golgi apparatus) in acini incubated for 60 minutes at 37°C in the absence (control, Co) or the presence (BFA) of 5 µM BFA. CD was detectable as red spots at the basal region of cells incubated in control medium (Co, arrows). A strong red labelling was also detectable in the basal region of acini incubated in the presence of BFA (arrows). In acini incubated in control medium, TGN38 (green) was located in the supranuclear region of cells (arrowheads) surrounding the aperture of the acinus which corresponds to the connection with the ductules (L). When acini were incubated in the presence of BFA, TGN38 was dispersed in the cells (arrowhead) confirming the disassembly effect of the toxin on the Golgi complex (BM, basal membrane; L, lumen). Bar, 10 µm. (B) Immunoblotting analysis of CD in cell homogenates (H) and conditioned media (CM) from acini incubated as above. The CD molecular forms are indicated (P, proCD; Msc, mature single-chain; LM, large chain of the mature double-chain). The enzymatic active mature single-chain CD form is detectable in medium from BFA-treated acini.
|
