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Fig. 6. C. elegans sdn-1 (ok449) mutants show loss of heparan sulfate proteoglycan immunoreactivity mainly in the nerve cords and vulva. Immunolocalization of heparan sulfate proteoglycans was carried out in whole worms as described for Fig. 3, for both wild-type (panels a and b) and sdn-1 (ok449) mutants (panels c and d). In sdn-1 (ok449) mutants, some immunoreactivity remained in the nerve ring shown in c (white arrowheads) but no staining was observed along the neural cords and the vulva (d). White arrows indicate the ventral nerve cord, gray arrows the dorsal nerve cord. All images are 3D reconstructions of image stacks. Bar, 20 µm.





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