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Fig. 1. The tsp-15 gene of Caenorhabditis elegans and its predicted product. (A) Schematic diagram of the tsp-15 genomic region, transgene, the relative position of the sv15 mutation, and results of rescue analyses. +, rescue; –, no rescue. (B) ClustalW comparison of TSP-15 (Q9XVM9) with human CD151 (P48509), mouse CD151 (Q35566) and fruit fly Tsp74F (Q9VVM5), which are the tetraspanins from other species that most closely resemble TSP-15. Identical and similar amino acids are indicated. The identities of the sequences between human and worm or fly and worm are both 24%. (C) Representation of the position of the sv15 mutation (a G-to-T transversion), and the effect of the mutation on splicing of intron 4. The mutation alters the splice donor site, and a cryptic splice donor in the middle of intron 4 is often used (upper versus lower case). (D) Schematic representation of the putative structure of TSP-15 and the relative position of the sv15 mutation. Well-conserved amino acid residues among the tetraspanin family are indicated. (E) Expression of the wild-type transcript is reduced in tsp-15(sv15) mutants and a cryptic donor site is often used. RT-PCR and subsequent Southern blotting of tsp-15 transcripts obtained from single mutant larvae. The region of tsp-15 examined by the RT-PCR is indicated with a double arrow in A. tsp-15(sv15) mutants produced a PCR product of the same length as that of the wild type and the ratio of correct and irregular transcripts differs for each individual mutant.

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