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Fig. 4. Expression pattern of TSP-15::GFP in C. elegans. (A-E) Confocal images of the expression pattern of TSP-15::GFP in transgenic animals. Expression begins in 1.5-fold embryos (A) and continues into adulthood, where it is seen in the pharynx, vulva and body wall (B). (C,D) A cryosectioned Ex[tsp-15::gfp] adult worm is shown. TSP-15::GFP is not seen in the body muscle quadrants, which are visualized by rhodamine-phalloidin staining (C), but is present in the hypodermis, as confirmed by colocalization with MH5 antigen (D, arrows), a component of the FOs. (E) A punctate localization of TSP-15::GFP (arrows) in thick region of hypodermis. Bars, 25 µm.





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