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Fig. 6. Cuticular and hypodermal abnormalities in tsp-15(RNAi) C. elegans. (A-D) Transmission electron micrographs of the epidermis of N2 and tsp-15(RNAi) animals. C and D are higher magnification images of tsp-15(RNAi) animals. In tsp-15(RNAi) animals, the cuticle is dissociated in the cortical layer (c). Unidentified material accumulates within the cortical and medial layers (m) of the cuticle (black arrows in B and C; compare with A), but the fiber layer (f) remains intact. There are some abnormal gaps within the hypodermis (asterisks in B and D). Note that the hypodermis and basement membrane are severely distorted, and the basement membrane has inconsistent thickness (C,D). The FOs, represented as electron-dense structures are indicated by white arrows. Cu, cuticle; Hy, hypodermis; BM, basement membrane; Mu, muscle. Bars, 200 nm.





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