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Fig. 8. Impairment of the membrane barrier function owing to reduction of tsp-15. (A-F) Living animals were incubated for 15 minutes with the membrane-impermeable fluorescent dye Hoechst 33258. N2, tsp-15(RNAi), and bli-2(e768) adult animals (A-C) and tsp-15(RNAi), tsp-15(sv15) and dpy-5(e61) larvae (D-F) are shown. Staining of nuclei by Hoechst 33258 is indicated with arrows. Although autofluorescence of gut granules is apparent, nuclear fluorescence is usually not observed within living N2, bli-2, or dpy-5 animals (A,C,F). In contrast, nuclear staining is frequently seen in the tsp-15(RNAi) and tsp-15(sv15) animals (B,D,E). Moreover, nuclear staining can be seen in tsp-15(RNAi) larvae that do not yet exhibit blisters (D). (G) The ratio of larvae that showed Hoechst-positive nuclei was determined for each genotype (n>100). Bars, 50 µm.





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