QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Chromatin immunoprecipitation (ChIP) analysis of HeLa cells transiently transfected with Orc1-Flag. (A) Schematic representation of the genomic region comprising the origin of DNA replication associated to the lamin B2 and TIMM 13 genes. The origin sequence is recognized by B48 primers. The control B13 region is also indicated. (B) Crosslinked chromatin was immunopurified with anti-Flag ( ${alpha}$ -Flag) or with a control non-specific antibody ( ${alpha}$ -IgG). After reversion of crosslinking, immunopurified DNA was subjected to competitive PCR with B48 and B13 primer sets. Dilutions of the competitor DNA (see Materials and Methods) are indicated above each lane. The identity of the amplification bands is indicated on the right of each panel. The relative abundance of B48 and B13 sequences in the immunopurified DNA is determined by the ratio of genomic (G) to competitor (C) bands. (C) Histogram showing the enrichment of B48 over B13 sequences in the immunopurified DNA obtained with ${alpha}$ -Flag and ${alpha}$ -IgG antibodies. Bar indicates the variation seen in three independent experiments.

Return to article