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Fig. 4. Keratin aggregate formation begins in areas rich in hemidesmosome proteins. (A) Unstretched DM-EBS (KEB-7) keratinocytes stained with polyclonal antibody BL18 against keratin 5 (green) and monoclonal antibody 233 against BP180 (red). Hemidesmosome components are usually concentrated in regions lacking many desmosomes at the periphery. These regions (arrows) coincide with aggregate formation early in stretch as seen in Fig. 2. Bar, 10 µm. (B) Localisaton of keratin aggregates with hemidesmosomal and desmosomal components after 10 minutes stretch. Cells stretched for 10 minutes were scored for colocalisation of keratin aggregates with desmoplakin, BP180 or plectin. 300 cells were counted for each antibody and results expressed as the average number per 100 cells. This shows that after short periods of stretch keratin aggregates form more commonly in areas of the cell high in hemidesmosomal components.
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