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Fig. 6. Keratin rings are associated with components of hemidesmosome and desmosome junctions in cells with mutant keratin. Fragmentation of the mutant keratin network in response to stretch leads to small fragments of keratin associated with desmoplakin (A). Keratin fragmentation results in the formation of ring structures, which are intercalated with patches of desmoplakin (B). HD1/plectin associates with these ring structures and is particularly located at adhesion points between rings, associated with the formation of chains of rings (C). BP180 (D), plakoglobin (E), BP230 (F) and plakophilin (G) are also associated with keratin rings. The hemidesmosomal transmembrane protein, ß4 integrin, showed no specific localisation with keratin fragments or keratin rings (H and I). Single optical slices were obtained using deconvolution microscopy after 120 minutes of stretch. Cells were stained using polyclonal antibody BL18 against keratin 5 (green) and monoclonal antibodies against desmoplakin (11-5F), HD1/plectin (HD121), BP180 (233), plakoglobin (CBL175), BP230 (IE5), plakophilin (PP1-5C2) (all red) and ß4 integrin (CD104) (blue). Bars, 1 µm.





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