spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Freeze-fracture electron microscopy reveals assemblies of TJ fibrils in wild-type and mutant cystic EBs. (A) Low-magnification image of large portions of the plasma membrane of a cystic EB. TJ fibrils are seen arranged both as a continuous belt, separating the apical (am) and the basolateral (lm) membrane domains, and as spatially restricted focal arrays within the basolateral membrane. (B-D) Higher magnification views, showing similar TJ belts (three to five strands thick) in one wild-type (WT1) (B), one heterozygous (+/–) (1;166) (C) and one homozygous (–/–) (3;96) (D) EB clone. Similar results were obtained with other clones (Table 5). (E-G) Higher-magnification views, showing focal arrays of TJ fibrils, which segregate microdomains within the basolateral membrane in wild-type (E), heterozygous (+/–) (F) and homozygous (–/–) (G) EBs. The arrowheads point to microvilli. Scale bars, 0.5 µm (A), 225 nm (B-G).





Right arrow Return to article