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Fig. 2. Invasion of MDCK-C cells in precultured chick heart fragments and inhibition of cell compaction. (A-D) Sections of confrontation cultures of MDCK-E clone A control cells (A,B) and MDCK-C clone W cells (C,D), fixed and stained with haematoxylin-eosin (A,C) or with antiserum against MDCK cells (B-D). Arrowheads in A and B highlight the epithelioid cell layer. Arrowheads in C and D mark invasive MDCK-C-EGFP clW cells. Bar, 100 µm. (E-H) Slow aggregation assay. Phase contrast images of MDCK-E clA control cells in the absence (E) or presence (F) of DECMA-1 antibody. (G,H) MDCK-C cl 3 (G) and MDCK-C cl W (H) in the absence of DECMA-1 antibody. Bar, 500 µm. (I) Fast aggregation assay. Plotted curves of relative volume distribution (y-axis) as a function of particle diameter (x-axis) are shown for MDCK-E clB without DECMA-1 antibody after 30 minutes ({circ}), MDCK-E clB with DECMA-1 antibody at 30 minutes ({triangleup}) and MDCK-C cl5 without DECMA-1 antibody at 30 minutes ({blacksquare}).





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