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Fig. 1. Cdr2p-GFP localizes to the medial region of cells. (A) Cdr2p-GFP was visualized in live cells (KGY681) grown in YE medium at 25°C. (B) Interphase and mitotic cells showing a broad or thin band of Cdr2p-GFP, respectively. In panels on the right, cells are rotated to illustrate cortical localization. (C) Cdr2p-GFP was visualized in live cells also expressing Sid4p-GFP (KGY961) as a spindle pole body marker to determine cell cycle stage. Cells representing different cell cycle stages are shown. Individual cells are outlined with gray lines. Indicated stages of cell cycle: a, interphase; b and c, metaphase; d and e, anaphase/telophase; f, next cell cycle.





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