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Fig. 3. Cdr2p is mislocalized following disruption of sterol-rich membrane domains. (A) cdr2-GFP (KGY681) cells were grown for 1 hour at 32°C in 5 µg/ml filipin, 10 µg/ml filipin or 0.01% DMSO as a solvent control. Cdr2p-GFP was visualized in live cells. Control cells grown in DMSO were treated with filipin for 1 minute and observed immediately. (B) cdr2-GFP cdc25-22 cells (KGY2773) were grown to mid-log phase at 25°C and shifted to 36°C for 3.5 hours. Cdr2p-GFP was visualized in live cells.





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