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Fig. 5. Cdr2p and septins are functionally independent. (A,B) spn4-deleted cells (KGY925) were transformed with pREP1 or pREP1cdr2+ and colonies that formed in the presence of thiamine were struck to plates with and without thiamine (A) or grown in liquid cultures in the absence of thiamine for 18 hours before DIC images were captured (B). (C) spn4-deleted cells (KGY4509) were grown to exponential phase in minimal medium and then washed into minimal medium lacking nitrogen. DIC images were captured of cells 48 hours later.





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