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Fig. 3. The HSR shows reproducible replication bands that depend on the HSR length. Clone-22 (A,B,E,F) and clone-24 cells (C,D,G,H) were analyzed in the same experiment as shown in Fig. 1H. The replication sites pulse-labeled in late S phase appeared as bands in the mitotic HSR. The arrows in A to D indicate the positions of the bands. The number of bands appeared to be proportional to the length of the HSR. A portion of the clone-22 and clone-24 cells had pairs of homologous HSRs. Such pairs within a single cell had similar replication bands (E-H). In E, the orientation of the HSR is shown as two arrows. For panels G and H, two microscope fields of the identical metaphase cell were digitally assembled to form one panel.





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