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Fig. 1. UNC-45 expression in the nematode Caenorhabditis elegans adult germline and early embryo. (A) A hermaphrodite gonad doubly stained with anti-UNC-45 antibody (green) and DAPI (blue), which stains the cell nuclei. (B) Distal part of hermaphrodite gonad doubly stained with pre-immune serum and DAPI as controls. (C,D) Two-cell (C) and 16-cell (D) embryos stained with anti-UNC-45. Arrows indicate concentration of UNC-45 at cell boundaries. In panels E-H, embryos are doubly stained for UNC-45 (E,G) and monoclonal MH27 (F,H), which recognizes an epitope associated with adherent junctions at the boundaries of hypodermal cells (Francis and Waterston, 1985; Hresko et al., 1994). As the embryo begins to elongate, the UNC-45 staining is progressively restricted to the presumptive muscle cells (G, arrowhead). (I,J) GFP and DIC images of a two-fold embryo transgenic for an UNC-45::GFP fusion protein, showing expression in the body wall muscles (arrowhead). (K-N) UNC-45 staining is detectable throughout embryogenesis whereas MHC-B staining is only detectable in the developing body wall muscles. K and M are stained with anti-UNC-45, L and N with anti-MHC B (5-8). (K,L) The same embryo at about 250 minutes after fertilization. (M,N) The same embryo at the 1.5-fold stage of elongation. The images of L and N were exposed for the same times, serving as controls to show that although MHC B does not show staining in the early embryos, UNC-45 does.

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